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Monday, December 23, 2013

Post-transcriptional gene silencing (PTGS).

1.0Introduction: Post written matterional agent silencing (PTGS), was starting developed in industrial sets in identify to control, plant size, colour manipulation in petunias a species of flower. And is now one of the hottest topics in molecular biology. It was by and by disc everywhereed that PTGS occurs in wildcat species as well as plants, and can be utilize to divine service research into viral defence and transposon silencing methods. It has now appeared that PTGS and in particular ribonucleic acid interference (RNAi) PTGS initiated by the introduction of double-stranded RNA (dsRNA), shut off the expression of peculiar(prenominal) genes in a variety of organisms (Cogoni et al., 2000). The technique involves using RNAi in a do were by double-stranded RNA induces the silencing of homologous endogenetic genes (Hammond et al., 2001). In the beginning, PTGS was head start used in plants over a decade ago. The first observation was made in petunias a species of flower (as mentioned above) by Jorgensen et al, (1996), trance trying to manipulate the plant by deepening the plants purple colour. But, the exit that he obtained was not a deeper purple colour, but kind of the opposite. The colour of the flowers appeared pale and eve white in slightly cases.
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Jorgensen then coined the observation Co suppression, because the suppression of two the introduced gene and homologous endogenous gene was suppressed. Jorgensen first pattern this to be a take associated with petunias, but was later found that this happens in more or less plant species, for example this has been flesh outd in fungi and was oddly present in Nuerospo! ra Carassa (Jorgensen et al., 1996). Although trans-gene-induced silencing in almost plants appears to involve gene-specific methylation this is transcriptional gene silencing (TGS), in other plants silencing occurs at the post-transcriptional train this is post-transcriptional gene silencing (PTGS). atomic run-on experiments in the later cases illustrate that the homologous transcript is made, but that it is rapidly...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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